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| 产品名称： | pGIKS-BioC |
|---|---|
| 商品货号： | TS188317 |
| Designations： | pGIKS-BioC |
| GenBank Number： | J04423 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | M Follettie |
| Applications： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector： | Construct size (kb): 3.63 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: KpnI Vector end: SacI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: Vector end (5): KpnI Vector end (3): SacI Excise insert: XhoI + SmaI Promoter giving the sense strand: T3 Promoter giving the antisense strand: T7 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Escherichia coli Gene symbol: bioC Gene name: biotin synthesis protein Contains complete coding sequence?: Y Type of DNA: genomic Insert 5 end: Modification: KpnI linker Insert 3 end: Modification: SacI linker Insert size (kb): 0.77 Cross references: DNA Seq. Acc.: J04423 Nucleotides 1-756 of the insert correspond to nucleotides 4190-4945 of J04423. Insert lengths(kb): 0.77 Gene product: biotin synthesis protein bioC Insert end (5): KpnI Insert end (3): SacI linker |
| Insert Size (kb)： | 0.770 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 4190-4945 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5 end): RsaI--0.14; SphI--0.48; SspI--0.65. The following enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--3.4; SmaI/XhoI--2.7, 0.78; XhoI--3.4. |
| Classification： | Enterobacteriaceae, Escherichia |