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| 产品名称： | pSE1076 |
|---|---|
| 商品货号： | TS188322 |
| Designations： | pSE1076 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 9.0000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 9.000 Type of vector: plasmid Cloning sites: Polylinker sites: Construction: pNKY51, Tn903 Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, kanR, URA3 replicon: pMB1 Vector: pSE1076 (plasmid) Construction: pNKY51, Tn903 Marker(s):URA3,ampR,kanR Construct size (kb): 9.0 Features: marker(s): ampR, kanR, URA3 replicon: pMB1 |
| Applications： | vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII/BamHI--5.2, 3.8; BamHI--9.0. Restriction digests of the clone give the following sizes (kb): BglII--9.0; BamHI--9.0; BamHI/BglII--5.2, 3.8; SmaI--9.0. Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, and selecting for stable integrants. A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains. A derivative of pNKY51 with kanR from Tn903 inserted at the SmaI site of pNKY51. The order of the major features in this plasmid is: BglII (nt 1) - hisG - nt 1150 - URA3 - nt 2350 - kanR - nt 3850 - hisG - BamHI (nt 5000) - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |