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| 产品名称： | pNK1207 |
|---|---|
| 商品货号： | TS188360 |
| Designations： | pNK1207 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | N Kleckner |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 8.0 Gene product: ATP phosphoribosyltransferase hisD |
| Insert Size (kb)： | 8.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.2 (doublet), 2.1; HindIII--10.5; PstI--10.5. Construct permitting translational fusions of lacZ to target genes. The transposase must be supplied in trans (pNK2882, ATCC 77226). LacZ is expressed after transpositions in the correct orientation and reading frame into an expressed target gene. Derived from pNK81 (ATCC 77337) by replacing 1.050 kb of uncharacterized DNA and the his promoter with 4 tandem repeats of the rrnB transcriptional terminator and replacing Tn10 with the mini-Tn1-LK. Contains the mini-Tn10-LK cassette that consists of the following features: IS10R - nt 63 - promoterless lacZ element starting at codon 8 - nt 3140 - kanR - IS10L - nt 4900. The order of the major features in this plasmid is: EcoRI - rrnB terminators - Tn10-LK in hisG - hisD - EcoRI - pMB1 ori - ampR. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561 |