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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS188370 |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | pYEGF8 AB103 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | produces protein epidermal growth factor urogastrone |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Type Strain： | no |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genotype： | MATalpha leu2-3 leu2-112 ura3-52 his4-580 pep4-3 |
| Preceptrol®： | no |
| Mating Type： | alpha |
| Ploidy： | haploid |
| Comments： | Produces epidermal growth factor
Produces functional product (assayed by receptor binding) of 6 kDa, indicating proper excision of the alpha factor leader sequence.
The 1.75 kb insert consists of the gene for the alpha mating factor in which a central portion has been replaced by the synthetic sequence encoding epidermal growth factor.
The order of the major features in this plasmid is: BamHI - MATalpha/EGF/MATalpha - BamHI - 2 micron ori - LEU2 - 2 micron ori - pMB1 ori - bla. DNA: Synthetic
Insert Lengths (kb): 1.75
Gene Product: epidermal growth factor (urogastrone) EGF
Alleles: A1, B1, B2, A2
Target Gene: epidermal growth factor |
| Medium： | ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Name of Depositor： | Chiron Corp., B Mendez, Chiron Corp. |
| U.S. Patent Number： | |
| References： | Brake AJ. Secretory expression in eukaryotes. US Patent 4,870,008 dated Sep 26 1989 |