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| 产品名称： | pTL-5495 |
|---|---|
| 商品货号： | TS188412 |
| Designations： | pTL-5495 |
| Species： | Tobacco etch virus |
| Depositors： | WG Dougherty |
| Vector： | Construct size (kb): 7.159999847412109 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.079999923706055 Gene product: VPg, 6 kDa |
| Insert Size (kb)： | 4.080 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | SalI does not excise the insert. There is an internal SalI site in the insert. Restriction digests of the clone give the following sizes (kb): BamHI--7.2; HindIII--3.3 (doublet) + other; SalI--4.9, 2.3; KpnI--7.2. The vector (pTL-8) allows in vitro expression of the insert as a fusion protein. The insert includes TEV cDNA sequences from nt 5413 to the 3 terminus of the genome at nt 9495. |
| Classification： | Potyvirus |
| References： | Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986. Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076 Carrington JC, Dougherty WG. Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J. Virol. 61: 2540-2548, 1987. |