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微信小章| 产品名称： | Human genomic DNA (MDA-MB-435S) |
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| 商品货号： | TS188433 |
| Product Format： | frozen Approx.10 µg |
| Storage Conditions： | -20°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Applications： | However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. |
| Comments： | This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line PubMed ID: 12354931 and PubMed ID: 17004106. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Introduction： | Cell Line Description: This cell line was originally described asxa0a spindle shaped variantxa0of the parentalxa0MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. However, recent studies have generated questions about the originxa0of the parent cell line, MDA-MB-435, and by extension HTB-129.xa0Gene expression analysis of the cells producedxa0microarrays in whichxa0MDA-MB-435 clustered with cell lines of melanoma originxa0instead of breast PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052. Additional studiesxa0havexa0since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line PubMed ID: 12354931 andxa0PubMed ID: 17004106. |
| Quality Control Specifications： | DNA Quality Control Information: ·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis ·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel ·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8 ·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion. |