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微信小章| 产品名称： | SNL76/7 |
|---|---|
| 商品货号： | TS188491 |
| Organism： | Mus musculus, mouse |
| Tissue： | Embryo |
| Cell Type： | Fibroblast |
| Product Format： | frozen |
| Morphology： | Fibroblast |
| Culture Properties： | Adherent |
| Biosafety Level： | 1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at
http://www.cdc.gov/biosafety/publications/bmbl5/index.htm. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Gender： | Male and female mixed |
| Strain： | SIM (Sandos Inbred Mice) |
| Applications： | This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells and induced pluripotent stem (iPS) cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | SNL76/7 was clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF) at an abundant level. |
| Clinical Data： | Male and female mixed |
| Comments： |
The STO cell line was transfected with a G418-resistance cassette, RV4.0 PubMed: 2822260 and a LIF expression construct PubMed: 3143916. Inclusion of G418 in the medium is not necessary for normal cell growth.xa0Note: ATCC tested that this cell line is resistant to:xa0G 418 (neomycin): 350 µg/mL. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:10
Interval: every 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.xa0 xa0
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| Cryopreservation： | Complete growth medium supplemented with an additional 35% fetal bovine serum and 10% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | A Bradley |
| Deposited As： | Mus musculus |
| Year of Origin： | 1988 |
| References： | McMahon AP, Bradley A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell. 62(6): 1073-1085, 1990. PubMed: 2205396 Takahashi K, et al. Induction of pluripotent stem cells from fibroblast cultures. Nat. Protoc. 2(12): 3081-3089, 2007. PubMed: 18079707 Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51(3): 503-512, 1987. PubMed: 2822260 Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916 Okita K, et al, Generation of germline-competent induced pluripotent stem cells. Nature 448(7151): 313-317, 2007. PubMed: 17554338 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |