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| 产品名称： | pTZA1 |
|---|---|
| 商品货号： | TS188495 |
| Designations： | pTZA1 |
| GenBank Number： | X13294 X66087 |
| Species： | Homo sapiens, human |
| Depositors： | J Golay |
| Applications： | contains sequence avian myeloblastosis viral (v-myb) oncogene homolog-like 1 |
| Vector： | Construct size (kb): 7.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTZ19 Intact vector size: 2.900 Type of vector: phagemid Vector end: BamHI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MYBL1 Gene name: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 Contains complete coding sequence?: Y Chromosome: 8; Localization: 8 q22 Tissue: B-lymphocytes Type of DNA: cDNA Insert 5 end: BamHI Insert 3 end: EcoRI Insert size (kb): 4.9 Cross references: DNA Seq. Acc.: X13294 Nucleotides 1-2317 of the insert correspond to nucleotides 25-2341 of X13294. DNA Seq. Acc.: X66087 Nucleotides 1270-4857 of the insert correspond to nucleotides 1-3587 of X66087. Insert lengths(kb): 4.900000095367432 Tissue: B lymphocyte Gene product: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 MYBL1 Target Gene: avian myeloblastosis viral (v-myb) oncogene homolog-like 1 |
| Insert Size (kb)： | 4.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.6, 3.0, 0.62, 0.35, 0.21, 0.19; EcoRI--3.6(doublet), 0.35, 0.23, 0.19; HindIII--4.8, 3.1; PstI--5.3, 2.4, 0.21; PvuII--4.3, 2.6, 1.1. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--1.13; BglII--1.74; EcoRI--0.64, 0.96, 1.11, 1.29; EcoRV--2.31; HindIII--3.11; KpnI--1.75, 3.45; NdeI--0.75; NcoI--1.31; PstI--2.40, 2.69; PvuII--0.93; SacI--2.35. There are internal EcoRI sites. The coding portion of the insert can be excised with HindIII. |
| References： | Golay J, et al. The human A-myb protein is a strong activator of transcription. Oncogene 9: 2469-2479, 1994. PubMed: 8058310 Josee Golay, personal communication |