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| 产品名称： | pMCT58 RW-146 |
|---|---|
| 商品货号： | TS188582 |
| Designations： | pMCT58 RW-146 |
| Species： | Homo sapiens, human |
| Applications： | Isolated from cMCT58 by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 5.849999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D1S77 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.15, 2.7; HindIII/EcoRI--3.15, 2.7; BamHI--5.6, 0.44; SalI--5.6; HindIII--5.6. The PvuII polymorphism has demonstrated codominant segregation in 60 families. Detection of the PvuII polymorphism (2 alleles) was verified by CEPH. Isolated from cMCT58 by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: MspI. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT58) on chromosome 1p D1S77. Nucleic Acids Res. 16: 9367, 1988. PubMed: 2902594 Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 |