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微信小章| 产品名称： | pRShGR pRShGRalpha |
|---|---|
| 商品货号： | TS188600 |
| Designations： | pRShGR pRShGRalpha |
| GenBank Number： | M10901 |
| Species： | Homo sapiens, human |
| Depositors： | Salk Institute for Biological Studies, RM Evans, Salk Institute for Biological Studies |
| Applications： | in another host, produces protein glucocorticoid receptor |
| Vector： | Construct size (kb): 6.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.799999952316284 Tissue: fibroblast Gene product: glucocorticoid receptor GRL Target Gene: glucocorticoid receptor |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.7, 1.35, 0.74; HindIII--5.8, 0.6, 0.37; KpnI--6.7; KpnI/DraI--2.8, 2.0, 0.74, 0.6, 0.45; KpnI/BamHI--6.7. KpnI/XhoI excises the insert with some polylinker; KpnI/DraI excises only insert. Contains nucleotides 26-2850 of the published sequence. The KpnI-XhoI fragment contains the entire coding sequence and 133 bp of pRSVcat. The orientation is 5KpnI to XhoI3. The DNA-binding domain of the protein extends from aa 421 to aa 486 (encoded from nt 1393 to nt 1590). The ligand-binding domain of the protein extends from aa 528 to aa 777. Expresses functional receptor when transfected into COS-1 cells. Restriction digests of the clone give the following sizes (kb): HindIII--5.8, 0.3, 0.16; KpnI--6.4; KpnI/XbaI--6.4; XhoI--6.4; XbaI--uncut. Detects mRNA of 5.6, 6.1, and 7.1 kb in human fibroblast cell line HT-1080. As described, the construct contains an SV40 ori, the RSV LTR, pBR322 sequences, and an SV40 polyadenylation signal. |
| References： | Giguere V, et al. Functional domains of the human glucocorticoid receptor. Cell 46: 645-652, 1986. PubMed: 3742595 Evans RM. The steroid and thyroid hormone receptor superfamily. Science 240: 889-895, 1988. PubMed: 3283939 Evans RM, et al. Retinoic acid receptor method. US Patent 4,981,784 dated Jan 1 1991 Hollenberg SM, et al. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318: 635-641, 1985. PubMed: 2867473 Hollenberg SM, et al. Colocalization of DNA-binding and transcriptional activation functions in the human glucocorticoid receptor. Cell 49: 39-46, 1987. PubMed: 3829127 Evans RM, et al. Hormone receptor-related bioassays. US Patent 5,071,773 dated Dec 10 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |