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微信小章| 产品名称： | 1G2 |
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| 商品货号： | TS188904 |
| Organism： | Homo sapiens, human |
| Tissue： | embryonic kidney |
| Cell Type： | epithelial; somatic cell hybrid |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 cells contain Ad5, SV40, and EBV viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Applications： | The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities).
The 1G2 cell line was deposited at the ATCC and is available as PTA-87. |
| Comments： | The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities).
The 1G2 cell line was deposited at the ATCC and is available as PTA-87. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Cells will slough off easily, subculture before confuency
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Bayer Corporation |
| References： | Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000 Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |