| 产品名称: | Chlamydia trachomatis |
|---|---|
| 商品货号: | TS189074 |
| Classification: | Chlamydiaceae, Chlamydia |
| Deposited As: | Lymphogranuloma venereum type III |
| Agent: | Chlamydia trachomatis |
| Strain: | Lymphogranuloma venereum (LGV III) strain 404 |
| Applications: | Sexually Transmitted Disease Research |
| Biosafety Level: | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format: | frozen |
| Storage Conditions: | -70°C or colder |
| Comments: | Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host: | CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA. Death of chicken embryo. |
| Recommended Host: | McCoy (ATCC® CRL-1696™) |
| Growth Conditions: | Recommendations for Infection: For best results in tissue culture, cells should be 24 to 48 hours old and 80% - 100% confluent. The cells will not continue to grow after adding the cycloheximide. Incubation:xa0 In TC, 2 to 3 days at 37°C, a 5% CO2xa0in air atmosphere is recommended. In CE, 7 to 10 days at 35°C. |
| Name of Depositor: | J Schachter |
| Source: | Lymph node from human with LGV |
| References: | Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618 Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529 |