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| 产品名称： | hIPL clone #4 |
|---|---|
| 商品货号： | TS189091 |
| Designations： | hIPL clone #4 |
| GenBank Number： | AF001294 |
| Species： | Homo sapiens, human |
| Depositors： | D Frank, B Tycko |
| Vector： | Construct size (kb): 3.727999925613403 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS+ Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: IPL Gene name: imprinted in placenta and liver Contains complete coding sequence?: Y Chromosome: 11; Localization: 11p15.5 Strain: Human Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 0.76 Cross references: DNA Seq. Acc.: AF001294 Nucleotides 1-760 of the insert correspond to nucleotides 1-760 of AF001294. Insert lengths(kb): 0.7599999904632568 Gene product: imprinted in placenta and liver IPL |
| Insert Size (kb)： | 0.760 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII/SacI--2.8, 0.9; HindIII--3.7. Insert contains the following restriction sites (approximate kb form the 5 end): ClaI--0.35kb; PstI--0.38kb, 0.58kb; NotI--0.44kb; BglII--0.47kb; XmnI--0.59; BamHI--0.61kb; XhoII--0.61kb. |
| References： | Qian N, et al. The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis. Hum. Mol. Genet. 6: 2021-2029, 1997. PubMed: 9328465 |