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| 产品名称： | pRB395 |
|---|---|
| 商品货号： | TS189202 |
| Designations： | pRB395 |
| Depositors： | R Bruckner, Universitat Tubingen |
| Biosafety Level： | 1 |
| Host： | Escherichia colixa0HB101 |
| Vector Information： | Size (kb): 6.5999999046325680
Vector: pRB395 (plasmid)
Promoters: Promoter vegII (B. subtilis)
Construction: pUB110, pBR322, cat, vegII
Marker(s): ampR,bleR,neoR
Features:
insert detection: cat marker(s): ampR, neoR, bleR promoter: vegII (B. subtilis) replicon: pUB110, pMB1 terminator: rrnB, to phage lambd |
| Applications： | expression vector shuttle vector terminator-cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; BamHI--6.8; BglI/BglII--3.5, 3.3. The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Terminator-cloning shuttle vector using the expression of chloramphenicol acetyltransferase as the reporter. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. The cat gene was derived from pUB112 by deletion of the promoter and the regulatory inverted repeat, resulting in constitutive cat gene expression under control of the vegII promoter. The order of the major features in the plasmid is: To terminator - vegII promoter - HindIII/MCS/EcoRI - cat - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028 |
| Shipped： | freeze-dried |