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| 产品名称： | Hypoxanthine - Aminopterin - Thymidine (H.A.T.) 500x concentrate |
|---|---|
| 商品货号： | TS189288 |
| Product Format： | 5 x 2 ml vials, each prepares 1 L of supplement |
| Storage Conditions： | Storage temperature of 2°C to 8°C is recommended. |
| Applications： | This reagent isxa0used in hybridoma production to select hybridomas from normal cell populations. HAT is supplied as five 2.0 mL vials. Each vial of concentrate can produce one liter of 1X HAT medium. |
| Comments： | It is supplied in 5 vials of 2 ml each; each vial is sufficient for 1 liter of supplement. Each vial contains: Hypoxanthine 13.610 mg Aminopterin 0.176 mg Thymidine 3.780 mg BSA fraction V 20.000 mg |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Quality Control Specifications： | The OM3-1.1 hybridoma cell line (ATCC HB-134™) was seeded in growth medium containing HAT.xa0 The population doubling time after two days of growth was ≤ 24 hours, indicating good growth, as expected for HAT resistant hybridoma cells.xa0 The P3/NSI/1-Ag4-1 (NS-1) plasmacytoma cell line (ATCC TIB-18™) was seeded in growth medium containing HAT.xa0 The cell concentration after 2 days of growth showed ≤ 20% change, indicating that the cells were dying in the presence of HAT, as expected for HAT-sensitive plasmacytoma cells. |