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| 产品名称： | pFa6a-KANMX6-pADH1 |
|---|---|
| 商品货号： | TS189319 |
| Designations： | pFa6a-KANMX6-pADH1 |
| Depositors： | DJ DeMarini |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH10B |
| Vector Information： | Size (kb): 4.6479997634887700 DESCRIPTION OF VECTOR: Intact vector size: 4.648 Type of vector: plasmid Cloning sites: PacI PmeI BglII Polylinker sites: Construction: pCRTOPO2.1 Features (with orientation and position when available): Vector: pFa6a-KANMX6-pADH1 (plasmid) Construction: pCRTOPO2.1 Marker(s):ampR Construct size (kb): 4.64799976348877 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--4.6; SalI/BglII--3.9, 0.7; EcoRI--4.6. The marker and promoter are flanked by the F4 primer site (5) and the R6 primer site (3). The size of the F4/R6 PCR product should be 2005 nt. F4 primer sequence: 5-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3. R6 primer sequence: 5-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3. Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899 |
| Related Products： | component of:ATCC 87819 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |