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| 产品名称： | pPbac |
|---|---|
| 商品货号： | TS189437 |
| Designations： | pPbac |
| Depositors： | BS Mroczkowski |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.6300001144409200 Vector: pPbac (phagemid) Promoters: Promoter polyhedrin Construction: pJVP10Z Marker(s):ampR Construct size (kb): 13.63000011444092 Features: marker(s): ampR other: 5-SmaI-Stuffer fragment-BamHI-3 promoter: P10 promoter: polyhedrin replicon: f1 signal peptide coding region: human placental alkaline phosphatase coding sequence: lacZ |
| Applications： | expression vector vector permitting visual detection of recombinants vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--14.0; HindIII--8.0, 4.3, 1.0, 0.9. The gene of interest can be inserted into pPbac by removal of the stuffer fragment which has a 5 SmaI site and a 3 BamHI site. pPbac is a baculovirus transfer vector that facilitates secretion of recombinant proteins in insect cell systems based on the presence of the human placental alkaline phosphatase leader signal. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mroczkowski BS, et al. Secretion of thermostable DNA polymerase using a novel baculovirus vector. J. Biol. Chem. 269: 13522-13528, 1994. PubMed: 8175786 |
| Shipped： | freeze-dried |