宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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LH31

货号 TS189439
中文名称 null
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产品名称: LH31
商品货号: TS189439
Designations: LH31
GenBank Number:

L06419

Species: Homo sapiens, human
Depositors: T Hautala
Vector:
Construct size (kb): 6.760000228881836
Insert:
DNA: cDNA
Insert lengths(kb): 2.407000064849854
Tissue: placenta
Gene product: procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase) LLH
Insert Size (kb): 2.407
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.5; PstI--3.75, 1.5, 0.95, 0.7; BglI--2.6, 2.3, 1.7; BglII--5.9, 0.9; PvuII--3.5, 2.7, 0.6.
The insert contains the following restriction sites (bp from the 5 end): BglI--819; BglII--540, 1390; HincII--2275; NcoI--706; PstI--732, 1383, 2330; PvuII--526, 1107; SacI--324, 378; SmaI--317.
Corresponds to nt 214-2620 of the published sequence and contains the complete coding region except for the first four amino acids.
Detects a single band of approximately 3.2 kb in Northern blots of RNA from human skin fibroblasts.
References:

Hautala T, et al. Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3----p36.2. Genomics 13: 62-69, 1992. PubMed: 1577494