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| 产品名称： | Human rhinovirus 37 |
|---|---|
| 商品货号： | TS189466 |
| Classification： | Picornaviridae, Rhinovirus, Human rhinovirus B |
| Agent： | Human rhinovirus 37 |
| Strain： | 151-1 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Presumed from human throat washings; this preparation has been derived from VR-1147 following treatment to remove mycoplasma. |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Genome Sequenced Strain： | Yes |
| Comments： | This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-169-001-021 (ATCC VR-1147). It replaces VR-1147 and VR-510, which are no longer available. Strain 151-1 is the prototype strain of Human rhinovirus 37 and it has been fully sequenced (GenBank: EF173423.1). |
| Effect on Host： | Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: H1HeLa (ATCC CRL-1958) Production Host: H1-HeLa (ATCC® CRL-1958x99) Alternate Hosts: HeLa (ATCC CCL-2), WI-38 (ATCC CCL-75), KB (ATCC CCL-17) and other human cells. |
| Growth Conditions： | For best results, plate cells 24-48 hours in advance. Infect at 80% - 90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible. |
| Name of Depositor： | NIAID, ATCC |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054 |