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| 产品名称： | pRS319a |
|---|---|
| 商品货号： | TS189467 |
| Designations： | pRS319a |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.3059997558593800 Vector: pRS319a (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS315 Marker(s):LEU2,ampR,CAN1 Construct size (kb): 10.30599975585938 Features: marker(s): CAN1 marker(s): LEU2 marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: SacI...KpnI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.8, 2.6, 1.1; HindIII--6.6, 2.8, 1.4; PstI--9.5, 1.0. pRS319a differs from pRS319 in that pRS319 has a truncated CAN1 gene and pRS319a has a full length CAN1 gene. A plasmid shuffling vector. YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains CAN1 marker (full length CAN1 gene) for negative selection. pRS319a was constructed by inserting a 4.161 kb PvuII-Sma CAN1 fragment from M3093 (CAN1 in pBluescript II) into the HpaI site (position 2175) of pRS315 (ATCC 77144). The order of the major features in this plasmid is: LEU2 - CAN1 - f1 ori - T7 promoter - lacZ/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4. |
| References： | Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795 |
| Related Products： | component of:ATCC 87562 |
| Shipped： | frozen |