微信小陈
微信小章
产品简介
购买须知
| 产品名称： | p425 TEF |
|---|---|
| 商品货号： | TS189468 |
| Designations： | p425 TEF |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.482 Vector: p425 TEF (plasmid) Promoters: Promoter for expression TEF Construction: pRS425 Marker(s):LEU2,ampR Construct size (kb): 7.482 Features: marker(s): LEU2 marker(s): ampR promoter for expression: TEF replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.0, 2.4; SacI/XbaI--3.8, 3.2, 0.42. High copy number shuttle expression vector. this vector has the TEF promoter for expression, the LEU2 marker gene and the 2 micron replicon. It is distributed in E. coli. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |