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| 产品名称： | pHER2-436-1 plasmid in E. coli |
|---|---|
| 商品货号： | TS189494 |
| Designations： | pHER2-436-1 plasmid in E. coli |
| Depositors： | A Ullrich |
| Vector： | Total size of construct (kb): 4.2 Excise Insert: ExoRI or SalI Vector: pUC12 Vector type: plasmid Vector size (kb): 2.7 Markers: ampR |
| Insert： | Insert size (kb): 1.45 Gene: Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog, ERBB2 DNA: cDNA Source: Human |
| Insert Size (kb)： | 1.450 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; BamHI--4.1, 0.4; HindIII--4.3; PstI--3.1, 1.0, +2 smaller; SalI--2.7, 1.5; SacI--2.65, 1.5. This is the 5 EcoRI fragment of lambdaHER2-436 which begins about 150 bp upstream of the initiation codon and encodes the first 433 amino acids--including the signal sequence and 4 potential glycosylation sites. |
| References： | Coussens L, et al. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230: 1132-1139, 1985. PubMed: 2999974 Tal M, et al. Human HER2 (neu) promoter: Evidence for multiple mechanisms for transcriptional initiation. Mol. Cell. Biol. 7: 2597-2601, 1987. PubMed: 3039351 |