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| 产品名称： | pfd-Tn5 pfdA31-Tn5 |
|---|---|
| 商品货号： | TS189634 |
| Designations： | pfd-Tn5 pfdA31-Tn5 |
| Depositors： | K Geider |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.3999996185302730 Vector: pfd-Tn5 (plasmid) Construction: pfdA3, Tn5 Marker(s):ampR,kanR Construct size (kb): 8.399999618530273 Features: marker(s): ampR, kanR replicon: fd |
| Applications： | integrating vector vector permitting positive selection for integration vector useful for transposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.5, 2.0; BamHI--4.4, 4.0; HindIII--5.0, 3.4; PstI--2.45 (doublet); 1.5, 0.96, 0.84. Suggested conditions for electroporation in these bacterial strains: E. coli C600Sm, fields strength 20 kV/cm, pulse length 5 to 12 msec; Erwinia amylovora Ea7/74Sm, 25 kV/cm, 5 msec; E. amylovora Ea1/79Sm, 12.5 kV/cm, 17.5 msec. To propagate the plasmid, the host must provide the bacteriophage fd gene 2 product. Suicide plasmid for transposon-mediated or site-directed mutagenesis of Erwinia amylovora and Escherichia coli after electroporation. The order of the major features in this plasmid is: EcoRI - ampR - fd ori - EcoRI - Tn5/kanR (6.5 kb). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Metzger M, et al. Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells. Nucleic Acids Res. 20: 2265-2270, 1992. PubMed: 1317549 |
| Shipped： | freeze-dried |