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| 产品名称： | pBAD30 |
|---|---|
| 商品货号： | TS189636 |
| Designations： | pBAD30 |
| Depositors： | L Guzman, J Beckwith |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.923 Vector: pBAD30 (phagemid) Promoters: Promoter araC Construction: pBAD28 Marker(s):ampR,araC Construct size (kb): 4.923 Features: marker(s): ampR marker(s): cmlR operator: I2 + I1 operator: O1 operator: O2 other: CAP site promoter: araC promoter for expression: arabinose BAD regulator: araC replicon: M13 replicon: p15A MCS: NheI...HindIII transcription terminator: rrnB T1 + T2 |
| Applications： | expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--2.0, 1.85, 1.2; BamHI--5.0; PstI--4.0, 1.1. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. The following primers can be used for sequencing of cloned inserts: 5 primer (27 - 8 bp upstream of the NheI site) 5-CTGTTTCTCCATACCCGTT-3; and one of two 3 primers: 3 primer 1 (2 - 19 bp downstream of the HindIII site) 5-CTCATCCGCCAAAACAG-3; 3 primer 2 (17 - 33 bp downstream of the HindIII site) 5-GGCTGAAAATCTTCTCT-3. Plasmid copy number is low due to the p15A replicon. This vector can be used when reduced gene expression is desirable. Plasmid contains the 3 portion of the cat (cmlR) gene which could promoter recombination if this plasmid is used in combination with other compatible cmlR plasmids. Plasmid is compatible with pBR-derived plasmids and may be used for coexpression of cloned inserts. Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of E. coli containing the plasmid. |