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| 产品名称： | REP4X |
|---|---|
| 商品货号： | TS189649 |
| Designations： | REP4X |
| Depositors： | SL Forsburg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.5000000000000000 Vector: REP4X (phagemid) Promoters: Promoter for expression nmt1 (full strength) Construction: pUC119 Marker(s):ampR,ura4+ Construct size (kb): 8.5 Features: marker(s): ampR marker(s): ura4+ promoter for expression: nmt1 (full strength) replicon: ars1 replicon: pMB1, f1 MCS: XhoI...SmaI |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--8.5; HindIII--5.5, 1.9, 1.1; EcoRI--7.1, 1.4. REP3X (ATCC 87603) and REP4X (TS189649) differ in that REP3X contains LEU2 (2.2 kb) and REP4X contains ura4+ (1.8 kb). REP3X (ATCC 87603) and REP4X (TS189649) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**) due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993). REP4X was constructed by deleting the ATG in the polylinker of the original REP4 plasmid. An XhoI linker was cloned into the blunted BalI-SalI cut REP4 plasmid. The SalI site is preserved. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516 Susan L Forsburg, personal communication |
| Related Products： | component of:ATCC 87685 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried E. coli containing the phagemid |