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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS189662 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | D3-157 |
| Application： | Transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- guaB22 rpsL125 fol-200 |
| Type Strain： | no |
| Comments： | Grows slowly because of reduced initiation of translation from the deficiency of N-formylmethionyl-tRNA-F. If revertants are present, they grow more rapidly, producing large colonies.
Host for plasmids containing dihydrofolate reductase genes.
A useful host for plasmids containing dihyrofolate reductase genes. |
| Medium： | ATCC® Medium 2957: LB Medium with 50µg/ml Thymidine and 100µg/ml Streptomycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | R Ferone |
| References： | Singer S, et al. Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli. J. Bacteriol. 164: 470-472, 1985. PubMed: 3900045 |