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| 产品名称： | p2-47-4 |
|---|---|
| 商品货号： | TS189698 |
| Designations： | p2-47-4 |
| Species： | Turnip crinkle virus |
| Depositors： | AE Simon |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.699999988079071 Gene product: satellite RNA C |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal SmaI, NcoI, DraI, SpcI and BstEII sites. Restriction digests of the clone give the following sizes (kb): BamHI--3.4; HindIII--3.4; EcoRI--3.4; HindIII/EcoRI--3.2, 0.70. The insert can be transcribed in vitro to yield infectious satellite cRNA. Contains a head to tail dimer of RNA C cDNA. |
| Classification： | Carmovirus |
| References： | Simon AE, Howell SH. Synthesis in vitro of infectious RNA copies of the virulent satellite of turnip crinkle virus. Virology 156: 146-152, 1987. PubMed: 3811230 Simon AE, Howell SH. The virulent satellite RNA of turnip crinkle virus has a major domain homologous to the 3 end of the helper virus genome. EMBO J. 5: 3423-3428, 1986. Anne E Simon, personal communication |