| 产品名称: | pHRnX0.8 |
|---|---|
| 商品货号: | TS189764 |
| Designations: | pHRnX0.8 |
| GenBank Number: | K01169 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 3.5 |
| Insert: | DNA: genomic Insert lengths(kb): 0.800000011920929 Gene product: renin REN Alleles: H1, H2, J1, B1, B2, F1, F2, G1, K1, K2, G2, I1 |
| Insert Size (kb): | 0.800 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments: | Restriction digests of the clone give the following sizes (kb): XbaI--2.7, 0.8; HindIII--3.5; BamHI--3.5; PstI--3.5. This probe contains exon 1. BglI, HindIII, BglII, TaqI, RsaI, and BstXI RFLPs are detected with a mix of the inserts from pHRnX0.8 (ATCC 57174), pHRnX3.6 (ATCC 57178), and pHRnES1.9 (ATCC 57176), apparently from base changes not within the coding regions of the gene. Enzyme(s) not detecting polymorphism: EcoRI, BanI, MlnI, BstEII, HphI, MspI, SstI, DraI, PvuII, MboII, ApaI, SinI, NciI, ScaI, XbaI, HinfI, NdeI, HincII. XmnI and PstI give uninterpretable results. Tested on a small sample. |
| References: | Naftilan AJ, et al. A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension 14: 614-618, 1989. PubMed: 2573574 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Hobart PM, et al. Human renin gene: Structure and sequence analysis. Proc. Natl. Acad. Sci. USA 81: 5026-5030, 1984. PubMed: 6089171 John M Chirgwin, personal communication |