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| 产品名称： | pMMB66HE |
|---|---|
| 商品货号： | TS189850 |
| Designations： | pMMB66HE |
| Depositors： | E Lanka |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.8199996948242190 Vector: pMMB66HE (plasmid) Promoters: Promoter tac Construction: RSF1010, pKK223-3, M13mp9 polylinker, lacIq Marker(s):ampR Construct size (kb): 8.819999694824219 Features: marker(s): ampR promoter: tac replicon: RSF1010, oriV, oriT repressor gene: lacIq transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Contains the following sites separated by (bp): EcoRI-739 -AhaIII-97 -ScaI-112 -PvuI-2101 -AccI-1848 -ScaI-55 -AccI-2039 -BstEII-372 -PvuII-93 -PvuII-94 -HpaI-320 -BstEII-182 -MluI-771 -EcoRI. Restriction digests of the clone give the following sizes (kb): AccI--3.8, 3.1, 1.9; BamHI--8.6; HindIII/PvuII--7.4, 1.3. In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter. An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. A 1246 bp lacIQ HindIII fragment inserted at XmaIII/BamHI of pKK223-3. A M13mp9 polylinker replaced the M13mp8 polylinker of pMMB66EH (ATCC 37620). THe 3005 bp NruI/AhaIII fragment was cloned between PstI (7768) and PvuII (1951) of RSF1010. The sequence of RSF1010 is given in this reference. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Scholz P, et al. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75: 271-288, 1989. PubMed: 2653965 Erich Lanka, personal communication |
| Shipped： | freeze-dried |