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| 产品名称： | p1-101 |
|---|---|
| 商品货号： | TS189874 |
| Designations： | p1-101 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4799999892711639 Gene product: DNA Segment, single copy D10S4 Alleles: A1, A2, B1, C1, B2, C2, A1, A2, B1, C1, B2, C2 |
| Insert Size (kb)： | 0.480 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 0.48, HindIII--5.8; PstI--6.1; SalI--uncut; BamHI--6.1. Nick-translated insert (sp. act >2x10 E8 dpm/ug) works well with ca. 1-2 x 10 E6 dpm/ml. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Codominant segregation demonstrated in 20 informative families (63 children) for locus A; for loci B and C, co-dominant segregation demonstrated in 3 families (23 children). Use standard hybridization and wash conditions. Insert gives more consistent results than entire plasmid. 10 E7 dmp/ml of oligolabeled insert (sp act >10E9 dpm/ug) gives clean autorads after 2-5 days exposure. This probe is derived from cosmid 3-3B. |
| References： | White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325 Litt M, et al. A single copy subclone, p1-101, from cosmid 3-3B, defines three RFLPs on 10pter-q23 HGM9 no. D10S4. Nucleic Acids Res. 15: 2783, 1987. PubMed: 2882480 Nakamura Y, et al. A primary linkage map of chromosome 10. Cytogenet. Cell Genet. 46: 667, 1987. Michael Litt, personal communication |