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| 产品名称： | pWS5 |
|---|---|
| 商品货号： | TS189879 |
| Designations： | pWS5 |
| GenBank Number： | J03020 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Vector： | Construct size (kb): 5.480000019073486 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.609999895095825 Gene product: segment S5, P5 polypeptide |
| Insert Size (kb)： | 2.610 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal EcoRV, BamHI, AccI and HincII sites. Restriction digests of the clone give the following sizes (kb): SmaI--uncut; BamHI--4.0, 1.8; EcoRI--5.6; HindIII--5.6; PstI--5.6; EcoRI/HindIII--3.0, 2.7. Linearize the plasmid with HindIII for in vitro transcription. This is a full-length clone of virus segment S5. The cDNA was tailored to have a 3-terminal EcoRV site before cloning into the SmaI site of pGEM-1. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 |