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| 产品名称： | p426 CYC1 |
|---|---|
| 商品货号： | TS190019 |
| Designations： | p426 CYC1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.244 Vector: p426 CYC1 (plasmid) Promoters: Promoter for expression CYC1 Construction: pRS426 (ATCC 77107) Marker(s):URA3,ampR Construct size (kb): 6.244 Features: marker(s): URA3 marker(s): ampR promoter for expression: CYC1 replicon: 2 micron MCS: SalI...XBAI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.75, 0.35; XbaI--3.4, 2.9; EcoRI--6.3. Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid. |