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| 产品名称： | pLA2920 |
|---|---|
| 商品货号： | TS190059 |
| Designations： | pLA2920 |
| Depositors： | Gray Freshwater Biological Institute; Univ. of Minnesota, RS Hanson |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 20.0000000000000000 Vector: pLA2920 (cosmid) Promoters: Promoter none Construction: pRK290, pMF517 cos Marker(s):tetR Construct size (kb): 20.0 Features: marker(s): tetR promoter: none replicon: oriV enhancer: none |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--20.0; HindIII--20.0; BglII--20.0; EcoRI--17.0; 2.6. This vector transfers efficiently to a broad host range including Escherichia coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas AM1, Pseudomonas putida, and Pseudomonas aeruginosa. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is a cosmid with a broad host range that can be used to characterize the genome of C1-utilizing microorganisms. This was constructed from pLA2917 (ATCC 39840) by deleting the kanamycin resistance segment from Tn5 to avoid internal rearrangement when the transposon moves and to permit selection of conjugants that have a Tn5 insertion. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Hanson RS, Allen LN. Methylotroph cloning vehicle. US Patent 4,824,786 dated Apr 25 1989 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |