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| 产品名称： | pUCSVK661-1 |
|---|---|
| 商品货号： | TS190073 |
| Designations： | pUCSVK661-1 |
| GenBank Number： | AF038616 |
| Species： | Simian virus 40, Simian virus 40 |
| Depositors： | JS Butel, JA Lednicky |
| Applications： | contains sequence in another host, produces protein |
| Vector： | Construct size (kb): 7.833000183105469 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: SV40 virus Gene name: DNA Segment Contains complete coding sequence?: Y Strain: rhesus monkey Tissue: brain, spleen, and lymph nodes Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 5.147 Cross references: DNA Seq. Acc.: AF038616 Nucleotides 1-5147 of the insert correspond to nucleotides 1-5147 of AF038616. Insert lengths(kb): 5.146999835968018 Tissue: brain, spleen, and lymph nodes |
| Insert Size (kb)： | 5.147 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.1, 2.7; BamHI--4.4, 3.4; SalI--7.8. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.44; BspHI--1.82, 2.68; EcoRI--1.69; HindIII--0.95, 1.4, 1.62, 3.38, 3.91, 5.08; NcoI--0.04, 0.24, 0.47; NdeI--3.71, 4.73; PstI--1.90, 3.11; PvuII--0.18, 1.62, 3.41. First protoarchetypal version of SV40 to be cloned and characterized. It has numerous nucleotide differences from SV40 reference strain 776. This is a viable clone (viral DNA, when excised from the plasmid using EcoRI, will form virus particles if the DNA is transfected into CV-1 cells or other permissive cells). Full-length genome of SV40 strain K661-1 (SV40-K661-1) was cloned into pUC-19 at the EcoRI site. |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685 |