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| 产品名称： | HFBCO78 |
|---|---|
| 商品货号： | TS190153 |
| Designations： | HFBCO78 |
| GenBank Number： | M85854 S76965 T05018 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.559999942779541 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene name: protein kinase inhibitor Contains complete coding sequence?: Y Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.6 Cross references: DNA Seq. Acc.: M85854; EST: EST02378; Primer: M13 Forward; THC id: THC169445 DNA Seq. Acc.: S76965; EST: EST02906; Primer: M13 Reverse; THC id: THC169445 Nucleotides 1-307 of the insert correspond to nucleotides 2079-1773 of S76965 with 98% identity. DNA Seq. Acc.: S76965; EST: EST02378; Primer: M13 Forward; THC id: THC169445 Nucleotides 1-235, 223-267, 260-312 of the insert correspond to nucleotides 505-739, 726-770, 762-824 of S76965. DNA Seq. Acc.: T05018; EST: EST02906; Primer: M13 Reverse; THC id: THC169445 Insert lengths(kb): 1.600000023841858 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: protein kinase inhibitor |
| Insert Size (kb)： | 1.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.1, 0.55. EST02378 matches GenBank accession MUSPKI with 91.48% identity over the sequence range: 1-235. This sequence record is for mouse cAMP-dependent protein kinase inhibitor. |
| References： | Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434 J C Venter, personal communication |