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| 产品名称： | EBO-pcD-hprt |
|---|---|
| 商品货号： | TS190201 |
| Designations： | EBO-pcD-hprt |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein hypoxanthine phosphoribosyltransferase |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.450000047683716 Tissue: fibroblast Gene product: hypoxanthine phosphoribosyltransferase HPRT Alleles: A1, A2, A3 Target Gene: hypoxanthine phosphoribosyltransferase |
| Insert Size (kb)： | 1.450 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | BamHI digestion yields 2 vector fragments (5.4, 5.2 kb) and the l.45 kb insert. Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--12.0; SfiI--7.4, 4.7; PstI--6.6, 3.2, 1.6; SacI--12.0; BamHI--5.2 doublet; 1.5; HindIII--8.0, 3.8; BglII--12.0. Vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. Expresses hprt gene and complements the HPRT-defect of UC lymphoblastoid cells. |
| References： | Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588 Jolly DJ, et al. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 80: 477-481, 1983. PubMed: 6300847 |