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| 产品名称： | pAR1219 |
|---|---|
| 商品货号： | TS190236 |
| Designations： | pAR1219 |
| GenBank Number： | J02518 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | Brookhaven National Laboratory, FW Studier, Brookhaven National Laboratory |
| Applications： | produces protein RNA polymerase |
| Vector： | Construct size (kb): 8.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: bacteriophage T7 Gene symbol: gene 1 Genomic copy number: unique Gene name: RNA polymerase Contains complete coding sequence?: U Type of DNA: genomic Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 4.44 Cross references: DNA Seq. Acc.: J02518 Insert lengths(kb): 4.440000057220459 Gene product: RNA polymerase gene 1 Target Gene: RNA polymerase |
| Insert Size (kb)： | 4.440 |
| Genotype： | pAR1219 T7gene1 lacI F- hsdR19 recA1 rpoB331 IN(rrnD-rrnE)1 lambda- |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4 (doublet); BglII-uncut; EcoRI--8.8; PstI--8.8; HindIII--8.8. The T7 RNA polymerase expression construct, encoding gene 1 and lac regulatory sequences, can easily be excised as a 4.4 kb BamHI fragment for insertion into another construct. The insert size given is for that of the sum of the lac and gene 1 sequences. Gene1 was sequenced from T7 wild-type DNA. The junctions between gene 1 and lac were sequenced. Lac sequence is taken from GenBank J01637. pAR1219 produces large amounts of T7 RNA polymerase in a suitable host upon induction with IPTG. Large amounts of active T7 RNA polymerase can be purified from the strain BL21/pAR1219. The lac fragment contains the lacI promoter, lacI gene, lacUV5 promoter, lac operator, and the translation start site and first 147 amino acids of B-galactosidase. pAR1219 was made by inserting a 1724 bp HincII fragment from pMC1, containing lac control elements, into the BglII site of pAR1173. The construction consists of a fragment containing the T7 RNA polymerase gene (gene 1, including nucleotides 3145 to 5841 of T7 DNA) under the transcriptional regulation of lacI and the lacUV5 promoter. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Studier FW, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. US Patent 4,952,496 dated Aug 28 1990 Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130, 1986. PubMed: 3537305 Davanloo P, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 81: 2035-2039, 1984. PubMed: 6371808 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |