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| 产品名称： | p425 CYC1 |
|---|---|
| 商品货号： | TS190249 |
| Designations： | p425 CYC1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 7.3670001029968260 DESCRIPTION OF VECTOR: Intact vector size: 7.367 Type of vector: plasmid Cloning sites: SalI HindIII PstI SmaI BamHI SpeI Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI_ Other unique sites: SacI Construction: pRS425 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: SalI...XbaI, ->, 3394-3452 promoter for expression: CYC1, marker(s): ampR, replicon: 2 micron, ->, 5920-7260 Vector: p425 CYC1 (plasmid) Promoters: Promoter for expression CYC1 Construction: pRS425 Marker(s):LEU2,ampR Construct size (kb): 7.367000102996826 Features: marker(s): LEU2 marker(s): ampR promoter for expression: CYC1 replicon: 2 micron MCS: SalI...XbaI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9, 3.2, 0.35; XbaI--3.9, 3.5; KpnI--6.0, 1.4. Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence. High copy number shuttle expression vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504 |
| Related Products： | component of:ATCC 87669 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |