| 产品名称: | Mouse mammary tumor virus |
|---|---|
| 商品货号: | TS190328 |
| Classification: | Retrovirus, Mammalian Type B Oncovirus |
| Agent: | Mouse mammary tumor virus |
| Strain: | RIII MT10 |
| Applications: | This holding is a cell line derived from an RIII mouse mammary tumor that produces MTV. Seed stock Hoechst stain positive--suggests contamination with mycoplasma. |
| Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation: | Mammary tumor (adenocarcinoma) from soft spontaneous tumor in 10-month-old RIII mouse |
| Product Format: | frozen |
| Storage Conditions: | Liquid nitrogen vapor |
| Comments: | This holding is a cell line derived from an RIII mouse mammary tumor that produces MTV. Seed stock Hoechst stain positive--suggests contamination with mycoplasma. Passage virus by passaging (splitting) cell line. Release of the virus into the supernate is facilitated by dispersing the cells and storing them at 4C for 7 days. |
| Effect on Host: | tumorigenicity: no effect in tissue culture |
| Recommended Host: | mouse |
| Growth Conditions: | Incubation: 2-3 days |
| Name of Depositor: | EY Lasfargues |
| Source: | Mammary tumor (adenocarcinoma) from soft spontaneous tumor in 10-month-old RIII mouse |
| References: | Lasfargues EY, et al. An established R3 mouse mammary tumor cell line; kinetics of mammary tumor virus (MTV) production. Proc. Soc. Exp. Biol. Med. 139: 242-247, 1972. PubMed: 4332331 |