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| 产品名称： | CD43-Afl3.6 |
|---|---|
| 商品货号： | TS190360 |
| Designations： | CD43-Afl3.6 |
| GenBank Number： | J04168 |
| Species： | Homo sapiens, human |
| Depositors： | J Frelinger |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.200000047683716 Tissue: peripheral blood cells (PHA activated) Gene product: sialophorin (gpL115, leukosialin, CD43) SPN |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains BamHI sites at positions 315 and 471 of the published sequence. Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; AvaI--3.1, 1.2; BamHI--4.1, 0.2; KpnI--4.2; XbaI--4.2. The insert extends from about position 248 to the AflII site at 1294 of the published sequence. The coding sequence extends form 96 to 1295. This partial cDNA encodes part of the extracellular and all of the transmembrane and intracellular domains. The insert detects mRNA of 8.0 kb and 2.3 kb in K562, Jurkat, HL-60, and U937 cell lines. The HincII site of the vector and the AflII site of the insert were destroyed in cloning. The EcoRI site is at the 5 end. Excision with AvaI cuts at the SmaI and XhoI sites of the polylinker. |
| References： | Pallant A, et al. Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16. Proc. Natl. Acad. Sci. USA 86: 1328-1332, 1989. PubMed: 2521952 John Frelinger, personal communication |