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微信小章| 产品名称： | Tetrahymena furgasoni Nanney and McCoy |
|---|---|
| 商品货号： | TS190399 |
| Deposited As： | Tetrahymena geleii Furgason |
| Strain Designations： | W SA13 |
| Application： | assay of arginine L-arginine assay of histidine L-histidine assay of lysine L-lysine assay of methionine assay of protein |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Woods Hole, MA, 1939 |
| Product Format： | test tube |
| Type Strain： | no |
| Comments： | species description magnetic fettering effect of molecular weight of protein on growth isoenzymic characterization of three mating groups bioassays of protein quality Osmoregulation food for suctorians electrophoretic characterization |
| Medium： | ATCC® Medium 357: Tetrahymena medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic |
| Cryopreservation： | 1.xa0 Transfer Tetrahymena from usual growth medium to ATCC Medium 1034 and allow to grow to near peak density. 2.xa0xa0 Harvest cells from a culture by centrifugation at 300 x g for 2 min.xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 3.xa0xa0 Adjust concentration of cells to 2 x 106/ml in fresh xa0xa0xa0xa0xa0 medium. 4.xa0xa0 While cells are centrifuging, prepare a 22% (v/v) sterile solution of sterile DMSO in fresh medium. a) Add 2.2 ml of DMSO to an ice cold 20 x 150 mm screw-capped test tube; b) Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.8 ml of ice cold medium; c) Invert several times to dissolve the DMSO; d) Allow to warm to room temperature. 5.xa0xa0 Add a volume of the DMSO solution equal to the cell xa0xa0xa0xa0xa0 suspension volume but add in 3 equal aliquots at 2 min xa0xa0xa0xa0xa0 intervals. Thus, the final concentration of the preparation xa0xa0xa0xa0xa0 will equal 11% (v/v) DMSO and 106 cells /ml. 6.xa0xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic xa0xa0xa0xa0xa0 screw-capped cryules (special plastic vials for xa0xa0xa0xa0xa0 cryopreservation). 7.xa0xa0 Place the ampules in a controlled rate freezing unit. The cooling cycle should be initiated no less than 15 min and no longer than 60 min after the addition of the DMSO to the cell preparation. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. Atxa0 -50°C ampules are plunged into liquid nitrogen. 8.xa0xa0 Store in the vapor or liquid phase of a nitrogen xa0xa0xa0xa0xa0 refrigerator. 9.xa0xa0 To establish a culture from the frozen state aseptically add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.xa0 Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule. 10.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm Petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant. xa0The cell suspension will pool at the edge of the plate. 11.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Continue to double the volume of the cell suspension at 10 minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v). xa0When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C.xa0 12.xa0xa0xa0xa0xa0xa0xa0xa0xa0 On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask. xa0Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of xa0the cell suspension. xa0Incubate the culture at 25°C. 13.xa0xa0xa0xa0xa0xa0xa0xa0xa0 After culture has been established subculture into fresh xa0xa0xa0xa0xa0 normal medium without sucrose.xa0 |
| Name of Depositor： | RP Hall |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1939 |
| References： | Borden D, et al. Electrophoretic characterization of classical Tetrahymena pyriformis strains. J. Protozool. 20: 693-700, 1973. PubMed: 4148695 Biol. Bull. 78: 9-23, 1940. Williams NE, et al. Protein similarities in the genus Tetrahymena and a description of Tetrahymena leucophrys n. sp.. J. Protozool. 31: 313-321, 1984. Font R, Ballentine R. Magnetic fettering of the ciliated protozoan Tetrahymena pyriformis. Trans. Am. Microsc. Soc. 95: 189-197, 1976. Hunter B, et al. Effect of molecular weight of protein on growth of Tetrahymena pyriformis. Nutr. Rep. Int. 24: 1043-1055, 1981. Nanney DL, et al. Isoenzymic characterization of three mating groups of the Tetrahymena pyriformis complex. J. Protozool. 27: 451-459, 1980. Rifkin JL. The role of the contractile vacuole in the osmoregulation of Tetrahymena pyriformis. J. Protozool. 20: 108-114, 1973. PubMed: 4347868 Henk WG, Paulin JJ. Scanning electron microscopy of budding and metamorphosis in Discophrya collini Toort. J. Protozool. 24: 134-139, 1977. PubMed: 405477 Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037 Stott JA, Smith H. Microbiological assay of protein quality with Tetrahymena pyriformis W. Br. J. Nutr. 20: 663-673, 1966. PubMed: 5957407 Dryden MJ, et al. Predicting protein digestibility and quality using an enzyme-Tetrahymena pyriformis W bioassay. J. Food Biochem. 1: 35-44, 1977. Evancho GM, et al. Comparison of Tetrahymena pyriformis W and rat bioassays for the determination of protein quality. J. Food Sci. 42: 444-448, 1977. |