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微信小章| 产品名称： | Metarhizium brunneum Petch |
|---|---|
| 商品货号： | TS190444 |
| Deposited As： | Metarhizium anisopliae (Metschnikoff) Sorokin var. anisopliae |
| Strain Designations： | M.a. 43 HRI 275.86 |
| Application： | Biological control of vine weevil, Otiorhynchus sulcatus |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Comments： | Compatibility with pesticides This fungus was re-identified as Metarhizium brunneum based on the multigene sequence analysis |
| Morphology： | On Blakeslee’s malt extract medium at 25°C after 15 days, two mycelial types were observed. White mycelium, cottony to floccose, dense. Yellowish tan mycelium velutinous to powdery with abundant dark brown to black sporodochium. Reverse creamy white to yellowish tan. Hyphae hyaline, guttulate. Conidia honey brown, elongated ovoid, smooth, single and in chains, 6-8 X 1.5-3 µm. |
| Medium： | ATCC® Medium 323: Malt agar medium ATCC® Medium 325: Malt extract agar (Blakeslees formula) ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
TCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTATCCAACTCCCAACCCCTGTGAATTATACCTTTAATTGTTGCTTCGGCGGGACTTCGCGCCCGCCGGGGACCCAAACCTTCTGAATTTTTTAATAAGTATCTTCTGAGTGGTTAAAAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGTCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTACGCCCCTCAAGTCCCCTGTGGACTTGGTGTTGGGGATCGGCGAGGCTGGTTTTCCAGCGCAGCCGTCCCTCAAATCAATTGGCGGTCTCGCCGTGGCCCTCCTCTGCGCAGTAGTAAAACACTCGCAACAGGAGCCCGGCGCGGTCCACTGCCGTAAAACCCCCCAACTTTTTATAGTTGACCTCGAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCCAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGTGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCACTTATGACCAGACTTGGCCCCGGTGAATCATCCAGCGGTTCCCGTTGGTGCACTTTGCCGGGGTTCAGGCCAGCATCAGTTCGCTCCGGGGGATAAAGGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCGCAATACCCTGTGGCGGGCTGAGGTTCGCGCTTTATGCAAGGATGCTGGCATAATGGTCATCAGTGAC Elongation factor 1-alpha gene TCACATCAACGTGGTCGTTATCGTAAGTCGCCTGCCTCCATTTCGAGCCTTGTAGAAGCTGTTATACTGACTTGCTTGTCGTAGGGGTATGTTTCGGAGCCTACACTCTTCGCCGTCTCGAGTTTGTGATAACTGACTGGTCCTCACAGCCACGTCGACTCCGGCAAGTCTACCACCACTGGTCACTTGATCTACCAGTGCGGTGGTATCGACAAGCGTACCATTGAGAAGTTCGAGAAGGTAAGCCAAACCACTCCGATTAATGATCTGCTATTGTTTGGCGATGAACACTATTGGGTTTCCCGCTGTCTGTCGGCCATTACCCCTCACTGTGACACGAAAATTTTCGCGGGGCCTTTATCTTGAACTTTGGTGGGGCACCATACCCCGCCAGCTGTCGAGGGTGTCTCTTTGTGTCTCTGGCTGTTGAAACCACAATATTGTCGTTGCTTTCAGAGGAAAAAACATTAAACTAATTTGGATCGCTGTATAGGAAGCCGCTGAACTCGGCAAGGGTTCCTTCAAGTACGCATGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATCGACATTGCCCTCTGGAAGTTCGAGACTCCCAAGTACTATGTCACCGTCATTGGTATGTCGACTTGCGCCAACTGACCGCATACTGTTCTCCCAAATTGAATGCTAATGCCCCTCCCACAGACGCTCCCGGTCACCGTGACTTTATCAAG beta tubulin gene CCCCCCTCCATTGTCTAGGACCCCTCCTCGACGCGTTCCATGGTCGTGCCCCTGATTTTGGTACCCCGCTGTATGAGGAGGCCACGTCCATATACGCACGCCCAACGGATCATCAGGACGAGGCAAAACGCGCTGCATCATCATAATGAACATTGGCTGACGATTTATTTCCTTTTGATCTTACACAGGTTCACCTTCAGACCGGTCAGTGCGTAAGTAAACAAATTCGCCGCTATTTTCGAATGAATTGTTACGGAGACCGGGAGCTTACAATATTTCGACAGGGTAACCAAATTGGTGCTGCTTTCTGGCAGACCATCTCTGGCGAACATGGCCTCGACAGCAATGGTGTCTACAACGGTACCTCTGAGCTCCAGCTCGAGCGTATGAGCGTCTACTTCAATGAGGTAAGTTTGAATGAGCCGAATCCTAGGATGTTGAGGAACCATAGGTTGTTATCCATGGGGAGATCAAAGACTGACGCGGGGACTCCCCTCAAAAGGCCTCCGGTAACAAGTATGTTCCTCGCGCCGTCCTTGTCGATCTTGAACCTGGCACCATGGATGCCGTCCGTGCCGGTCCTTTCGGTCAGCTTTTCCGTCCCGACAACTTCGTCTTTGGTCAGTCTGGTGCTGGCAACAACTGG |
| Name of Depositor： | G Zimmermann |
| Chain of Custody： | ATCC <-- G Zimmermann <-- E Muller-Kogler |
| Isolation： | Codling moth larvae, Cydia pomonella, Austria |
| References： | Moorhouse ER, et al. Influence of fungicides and insecticides on the entomogenous fungus Metarhizium anisopliae, a pathogen of the vine weevil, Otiorhynchus sulcatus. Biocontrol Sci. Technol. 2: 49-58, 1992. |