For optimal cell attachment, cell culture flasks should be prepared before thawing the cells.

1. Decontaminate the external surface of the 0.1% Gelatin Solution bottle by spraying with 70% ethanol.
2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, add 1.0 mL of 0.1% Gelatin Solution per 10 cm² of culture surface area (e.g., 2.5 mL if using a T-25 flask).xa0
3. Rock culture flask to coat surface; place in a 37°C incubator (with or without 5% CO₂) for at least 30 minutes, and up to overnight.
4. Aspirate the excess gelatin solution from the culture flasks using sterile technique.
5. Add 5.0 mL of complete growth medium per 25 cm² of culture surface area (e.g., 5.0 mL if using a T-25 flask).
6. Place the gelatin-coated flasks in a 37°C, 5% CO₂ incubator for at least one hour to equilibrate before inoculating with the cell suspension. xa0xa0