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微信小章| 产品名称： | pR16.11 purified plasmid DNA |
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| 商品货号： | TS190559 |
| Designations： | pR16.11 purified plasmid DNA |
| GenBank Number： | L24917 |
| Species： | HRV type 16 molecular clone |
| Depositors： | W Lee |
| Applications： | Contains full-lengthxa0sequence |
| Vector： | Construct Size (Vector + Insert) (kB): 9.460xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Host required: E. coli MV1193 Host medium: LB broth with 50 µg/mL ampicillin (ATCC Medium #1227) Type of vector:xa0 E. coli plasmid Name of vector:xa0 pUC19 + T7 promoter Vector size (kb):xa0 nucleotides 1 to 400 and 2678 to 2686 were deleted in the cloning process. Markers (ie. AMPR):xa0 AMPR Cloning site:xa0 BamHI |
| Insert： | Insert: Human rhinovirus (HRV) type 16 cDNA Classification:xa0 Picornavirus, Rhinovirus, Human Insert description (full-length, etc.):xa0 full-length HRV 16 cDNA Insert size (kb):xa0 7.124 kb + poly A tail Full/partial sequence available (ie. GenBank #): L24917, with differences noted (J. Virology 77: 6235-6244, 2003) Markers (genes and/or restriction sites): Enzymexa0 # sitesxa0 Fragment Lengthxa0 Location of Cuts BamH1xa0xa0xa0xa0xa0xa0xa0 2xa0xa0xa0xa0xa0xa0xa0xa0xa0 6455; 3005xa0xa0xa0xa0xa0xa0xa0xa0xa0 Pst1xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 3xa0xa0xa0xa0 6298; 2365; 527 Kpn1xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 3xa0xa0xa0xa0 4452; 4002; 1006 BgIIIxa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 4xa0xa0xa0 5085;2045;1864;466 |
| Insert Size (kb)： | 7.124 kb + poly A tail |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | 200 ng dried plasmid DNA |
| Comments： | This full-length infectious clone is not published yet although the sequence was published (Virus Gene 9:177-181, 1995). The first base of the HRV 16 genome was ligated to the last base of the T7 promoter. The HRV 16 genome has a poly (A) track of 52 As at the 3 end.xa0 The full-length T7 transcripts of HRV 16 generated from this construct had a specific infectivity of about 5 x 106 PFU/µg, which is similar to the virion RNA. The complete insert was found to differ from the GenBank sequence L24917 as follows (position in VRMC-8 sequence and base): 715 C, 2266 G, 2267 G, 2393 G, 2419 G, 2620 G, 2795 A, 3324 A, 3640 C, 3652 C, 4678 A, 4878 G, 4914 A, 5341 G, 6150 G, 6394 T, 6457 A, 6511 A. Ref |
| References： | Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955 Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441 Lee WM, et al. Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes 9: 177-181, 1995. PubMed: 7732663 Lee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280 |
Lee WM, Wang W. Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis. J. Virol. 77: 6235-6244, 2003. PubMed: 12743280