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| 产品名称： | pRS411 |
|---|---|
| 商品货号： | TS190730 |
| Designations： | pRS411 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC® 33694™) |
| Vector Information： | Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalIxa0XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRIxa0EcoRV ClaI SalI XhoI ApaI KpnI Construction: pRS401, pRS84 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): MET15, →, 421-1270 replicon: f1, ←, 1860-2316 promoter for in vitro transcription: T7, →, 2486-2505 MCS: KpnI...SacI, →, 2513-2615 promoter for in vitro transcription: T3, ←, 2632-2651 coding sequence: lacZ, ←, 2317-2676 promoter: lac, ←, 2721-2749 replicon: pMB1, 3075-3075 marker(s): ampR, ←, 3833-4693 centromere: CEN6, 4835-4951 replicon: ARS4, 4952-5353 Vector: pRS411 (phagemid) |
| Applications： | YC-type (centromeric) shuttle vectorxa0
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | Component of: ATCC 87476
Component of: ATCC 87529 |
| Shipped： | frozen |
| Shipping Information： | Plasmid in E. coli |