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| 产品名称： | p554 |
|---|---|
| 商品货号： | TS190771 |
| Designations： | p554 |
| Depositors： | Wisconsin Alumni Res. Fndn., WM Sugden, Wisconsin Alumni Res. Fndn. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 28.8239994049072300 Vector: p554 (plasmid) Construction: EBV, pKan-2 Marker(s):G418R,ampR Construct size (kb): 28.82399940490723 Features: marker(s): ampR, G418R replicon: pMB1, EBV oriP, oriLyt |
| Applications： | contains sequence nuclear antigen 2 contains sequence nuclear antigen leader protein expression vector in another host, produces protein nuclear antigen 2 in another host, produces protein nuclear antigen leader protein shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--23.0, 6.1; EcoRI--22.5, 3.5, 2.9; BamHI--17.0, 6.6, 3.45, 1.95; HindIII--28.8. For expression, foreign DNA should contain the gene of interest as well as a promoter and polyadenylation signals. Maintained as approximately 10 plasmid copies in eukaryotic cells that provide the EBNA-1 gene product in trans. This protein is produced in P3HR-1 cells (ATCC HTB-62). Epstein-Barr virus-based shuttle vector that can be maintained as a plasmid in cells expressing EBNA-1 or induced to replicate lytically by treatment with tetradecanoylphorbol acetate or introduction of a construct expressing BZLF-1. The virus particles can then be used to infect other eukaryotic cells. Encodes all sequences necessary for virus packaging, including the terminal repeats. The order of the major features of the plasmid is: HSV-1 Tk promoter - G418 neo HSV-1 Tk terminators - EcoRI - oriP - EBV genes - oriLyt - SalI - EcoRI - terminal repeats (TR) - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sugden WM, Hammerschmidt W. Lytic origin of replication for epstein-barr virus (EBV). US Patent 5,194,601 dated Mar 16 1993 |
| Shipped： | freeze-dried |