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| 产品名称： | p37-1-34 p1-34 |
|---|---|
| 商品货号： | TS190793 |
| Designations： | p37-1-34 p1-34 |
| Species： | Homo sapiens, human |
| Applications： | The 1.7 kb fragment can be used as a probe. Sau3A digests give 2.0 kb (from insert, use as probe) + other smaller fragments. The cosmid was digested with Sau3A1 and a 2.8 kb fragment was used to construct this clone. |
| Vector： | Construct size (kb): 6.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: DNA Segment, single copy D20S8 Alleles: A1, A2, B1, B3, B2 |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | HindIII/EcoRI digests give 3.0 kb (vector) and 1.7, 0.7, 0.4 kb (insert). The 1.7 kb fragment can be used as a probe. Sau3A digests give 2.0 kb (from insert, use as probe) + other smaller fragments. Restriction digests of the clone give the following sizes (kb): BglI--4.7, 2.0; PvuII--6.4; HindIII--6.4; EcoRI--5.08, 1.1, 0.7; HindIII/EcoRI--3.3, 2.2, 1.0, 0.7. Long gel runs are required to resolve RFLP alleles. Depositor suggests 1% gels, 20 hrs, ca 2 V/cm. This locus is in linkage equilibrium with that seen by the 20 kb EcoRI fragment probe of cosmid 1-37. The compound locus has a PIC of 0.54. Hybridizations are done in 50% formamide, etc. at 50C; wash at 0.1X SSC /0.1% SDS, 65-68C. Probe hybridizes weakly to repeat sequences in TaqI fragments larger than 7 kb. This was derived from cosmid 1-37 (ATCC 59010). The cosmid was digested with Sau3A1 and a 2.8 kb fragment was used to construct this clone. |
| References： | Litt M, et al. A polymorphic locus on the long arm of chromosome 20 defined by two probes from a single cosmid. Hum. Genet. 73: 340-345, 1986. PubMed: 3017843 Michael Litt, personal communication |