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| 产品名称： | pEMBLYe31 |
|---|---|
| 商品货号： | TS190827 |
| Designations： | pEMBLYe31 |
| Depositors： | G Cesareni |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.1000003814697270 Vector: pEMBLYe31 (phagemid) Construction: pEMBL9, (ATCC 37395), bacteriophage f1, fragment of CV13 Marker(s):LEU2,ampR Construct size (kb): 8.100000381469727 Features: insert detection: lacZ marker(s): ampR, LEU2 replicon: pMB1, f1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector permitting construction of fusion proteins vector permitting production of single-stranded DNA |
| Comments： | ssDNA-producing plasmid with polylinker in lacZ and marker selectable in yeast. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771 Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935 |
| Shipped： | freeze-dried |