| 产品名称： |
Endothelial Cell Growth Kit-VEGF |
| 商品货号： |
TS190844 |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： |
frozen 1 kit |
| Storage Conditions： |
-20°C (or -70°C for long-term storage) |
| Comments： |
The Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for human large vessel endothelial cells. The final concentration for each component in complete endothelial growth medium is as follows:
- rh VEGF: 5 ng/mL
- rh EGF: 5 ng/mL
- rh FGF basic: 5 ng/mL
- rh IGF-1: 15 ng/mL
- L-glutamine: 10 mM
- Heparin sulfate: 0.75 Units/mL
- Hydrocortisone: 1 µg/mL
- Ascorbic acid: 50 µg/mL
- Fetal bovine serum: 2%
|
| Complete Growth Medium： |
- Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
- Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
- Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
- Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
- Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
- Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.
Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), add the indicated volume for each component:xa0
|
Component |
| Volume： |
Volume |
| Sterility Tests： |
Final Concentration |
| Functional Tests： |
Bovine Brain Extract (BBE) |
| C of A： |
1.0 mL |
|
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