宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Human genomic DNA (HCC38)

货号 TS190898
中文名称 null
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产品简介
购买须知
产品名称: Human genomic DNA (HCC38)
商品货号: TS190898
Product Format: frozen 10 µg
Storage Conditions: -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction:

Cell Line Description:

This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.xa0 HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 EGP2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D.

Quality Control Specifications:

DNA Quality Control Information:

·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis

·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel

·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8

·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion.